Rabbit Polyclonal antibody to ASPSCR1Tug (Tether containing UBX domain for GLUT4), also known as ASPL, ASPSCR1, RCC17, UBXD9, UBXN9, was first identified as a chromosomal translocation partner for TFE3 in patients with Alveolar soft part sarcoma and contains an UBX-like domain in its C-terminal region. Tug is found to tether GLUT4 in intracellular vesicles and to release GLUT4 for cell surface translocation upon insulin stimulation . Stable Tug depletion or expression of a dominant negative form stimulates GLUT4 redistribution.
Immunogen
Peptide sequence around aa.303 ~307(P-Q-Q-E-Q) derived from Human ASPSCR1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
ASPCR1 ASPL ASPS FLJ45380 RCC17 TUG UBXD9 UBXN9 ASPSCR1 alveolar soft part sarcoma locus UBX domain protein 9 renal cell carcinoma gene on chromosome 17 tether containing UBX domain for GLUT4 renal cell carcinoma papillary 17 alveolar soft
Target
ASPSCR1
Molecular Weight(MW)
70-75 (note)
Application
Application
Western blot analysis of extracts from HL60 cells using ASPSCR1 Antibody .
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.