Application
Western blot analysis using Mouse TUG monoclonal antibody against NIH / 3T3 cell lysate.| Product Name | TUG Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASPL, RCC17, TUG, UBXD9, UBXN9, Alveolar soft part sarcoma chromosomal region candidate gene 1 proteinTUG, ASPL, ASPS, ASPCR1 |
| Product description | The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.1) Nature 2003,425:727 - 733. |
| Immunogen | Ni-NTA purified truncated recombinant TUG expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASPSCR1 |
|---|---|
| Alternative Names | ASPL RCC17 TUG UBXD9 UBXN9 Alveolar soft part sarcoma chromosomal region candidate gene 1 proteinTUG ASPL ASPS ASPCR1 |
Application
Western blot analysis using Mouse TUG monoclonal antibody against NIH / 3T3 cell lysate.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Mouse TUG monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
