Application
Flow cytometric analysis of Jurkat cells using ATXN1 mouse mAb ( green) and negative control (purple).Application
Western blot analysis using ATXN1 mAb against HEK293 (1) and ATXN1 (AA: 645-815)-hIgGFc transfected HEK293 (2) cell lysate.Application
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and lung cancer tissues (right) using ATXN1 mouse mAb with DAB stainingApplication
Immunofluorescence analysis of NTERA-2 cells using ATXN1 mouse mAb ( green). Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin| Product Name | Ataxin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [2F5] to Ataxin 1The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known. This locus has been mapped to chromosome 6, and it has been determined that the diseased allele contains 41-81 CAG repeats, compared to 6-39 in the normal allele. At least two transcript variants encoding the same protein have been found for this gene.Tissue specificity: Widely expressed throughout the body. |
| Immunogen | Purified recombinant fragment of human ATXN1 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 2F5 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| For WB: Use at 1/500 - 1/2000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ATX1 SCA1 D6S504E ATXN1 |
|---|---|
| Target | ATXN1 |
| Molecular Weight(MW) | 87 kDa (note) |
Application
Flow cytometric analysis of Jurkat cells using ATXN1 mouse mAb ( green) and negative control (purple).
Application
Western blot analysis using ATXN1 mAb against HEK293 (1) and ATXN1 (AA: 645-815)-hIgGFc transfected HEK293 (2) cell lysate.
Application
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues (left) and lung cancer tissues (right) using ATXN1 mouse mAb with DAB staining
Application
Immunofluorescence analysis of NTERA-2 cells using ATXN1 mouse mAb ( green). Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin| Application Notes | For WB: Use at 1/500 - 1/2000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4℃, for long term storage, store at -20℃. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
