Application
Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.Application
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Ataxin 3 antibody at 1:500 dilution.Application
Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.Application
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3 (+). 10% SDS-PAGE Ataxin 3 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Ataxin 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Ataxin 3 (ataxin 3)Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Ataxin 3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Chicken, Sheep, BovineRat (96%), Pig (99%), Chicken (88%), Sheep (98%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AT3 antibody ATX3 antibody JOS antibody MJD antibody MJD1 antibody SCA3 antibody ATXN3 antibody spinocerebellar ataxia type 3 protein antibody ataxin-3 antibody ataxin 3 variant h antibody olivopontocerebellar ataxia 3 antibody ataxin 3 variant |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (3) |
| Cellular Localization | Nucleus matrix |
Application
Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.
Application
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with Ataxin 3 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using Ataxin 3 antibody at 1:500 dilution.
Application
Ataxin 3 antibody detects Ataxin 3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 protein stained by Ataxin 3 antibody diluted at 1:250. Blue: Hoechst 33342 staining.
Application
Ataxin 3 antibody detects Ataxin 3 protein by western blot analysis. 293T mock transfected (-) or transfected with a construct expressing human Ataxin 3 (+). 10% SDS-PAGE Ataxin 3 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | mouse brain , human Ataxin3-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
