Application
Untreated (–) and treated (+) A431 whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody diluted at 1:5000.Application
Untreated (–) and treated (+) 293T whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody diluted at 1:5000.| Product Name | ATF1 (phospho Ser63) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATF1 (phospho Ser63) (activating transcription factor 1)This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human ATF1 (phospho Ser63). The exact sequence is proprietary. |
| Modification | p-Ser63 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | ATF1 (phospho Ser63) |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Nucleus |
Application
Untreated (–) and treated (+) A431 whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody diluted at 1:5000.
Application
Untreated (–) and treated (+) 293T whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF1 (phospho Ser63) antibody diluted at 1:5000.| Positive Control | 293T , 293T (25 μg/ml anisomycin treatment for 30 min) , A431 , A431(200 ng/ml EGF treatment for 30 min) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
