Application
Sample (30 µg of whole cell lysate) A: HeLa (control) B: HeLa treat tunicamycin (4ug/ml) 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ATF4 antibody detects ATF4 protein at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 2 μg/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | ATF4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATF4 (activating transcription factor 4 (tax-responsive enhancer element B67))This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Pig, Sheep, Rhesus Monkey, Chimpanzee, BovineMouse (83%), Pig (89%), Sheep (87%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CREB-2 antibody CREB2 antibody TAXREB67 antibody TXREB antibody ATF4 antibody DNA-binding protein TAXREB67 antibody cyclic AMP-responsive element-binding protein 2 antibody cAMP-responsive element-binding protein 2 antibody cAMP response element-bi |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cell membrane , Nucleus |
Application
Sample (30 µg of whole cell lysate) A: HeLa (control) B: HeLa treat tunicamycin (4ug/ml) 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ATF4 antibody detects ATF4 protein at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 2 μg/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
