Application
IHC-P analysis of rat spleen using ATF6 antibody at a dilution of 1:200.Application
IHC-P analysis of rat lung using ATF6 antibody at a dilution of 1:200.Application
IHC-P analysis of human lung cancer using ATF6 antibody at a dilution of 1:200.Application
IHC-P analysis of human lung using ATF6 antibody at a dilution of 1:200.Application
ICC/IF analysis of A549 cells using ATF6 antibody (green) and DAPI (blue).Application
WB analysis of extracts from various cell lines using ATF6 antibody.| Product Name | ATF6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATF6This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011] |
| Immunogen | C term -peptide of human ATF6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Recommended Starting Dilutions: For WB: Use at a dilution of 1:500-1:2000. For IHC-P: Use at a dilution of 1:50. For ICC/IF: Use at a dilution of 1:50 - 1:200. Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | activating transcription factor 6 ATF6A |
|---|---|
| Target | ATF6 |
| Molecular Weight(MW) | 75 kDa (note) |
Application
IHC-P analysis of rat spleen using ATF6 antibody at a dilution of 1:200.
Application
IHC-P analysis of rat lung using ATF6 antibody at a dilution of 1:200.
Application
IHC-P analysis of human lung cancer using ATF6 antibody at a dilution of 1:200.
Application
IHC-P analysis of human lung using ATF6 antibody at a dilution of 1:200.
Application
ICC/IF analysis of A549 cells using ATF6 antibody (green) and DAPI (blue).
Application
WB analysis of extracts from various cell lines using ATF6 antibody.| Application Notes | Recommended Starting Dilutions: For WB: Use at a dilution of 1:500-1:2000. For IHC-P: Use at a dilution of 1:50. For ICC/IF: Use at a dilution of 1:50 - 1:200. Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
