Application
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/mL.Application
Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL.Application
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/mL.| Product Name | ATF6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATF6This gene encodes a transcription factor that activates target genes for the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Although it is a transcription factor, this protein is unusual in that it is synthesized as a transmembrane protein that is embedded in the ER. It functions as an ER stress sensor/transducer, and following ER stress-induced proteolysis, it functions as a nuclear transcription factor via a cis-acting ER stress response element (ERSE) that is present in the promoters of genes encoding ER chaperones. This protein has been identified as a survival factor for quiescent but not proliferative squamous carcinoma cells. There have been conflicting reports about the association of polymorphisms in this gene with diabetes in different populations, but another polymorphism has been associated with increased plasma cholesterol levels. This gene is also thought to be a potential therapeutic target for cystic fibrosis. [provided by RefSeq, Aug 2011] |
| Immunogen | ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| WB: 0.5 - 1 ug/ml. ICC: 10 ug/ml. IF: 10 ug/ml: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATF6A activating transcription factor 6 |
|---|---|
| Target | ATF6 |
Application
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 ug/mL.
Application
Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 ug/mL.
Application
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 ug/mL.| Application Notes | WB: 0.5 - 1 ug/ml. ICC: 10 ug/ml. IF: 10 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
