Rabbit Polyclonal antibody to ATG10Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
Immunogen
ATG10 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ATG10.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAWB
WB: 0.5 - 2 ug/mL:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
APG10L autophagy related 10 pp12616 APG10
Target
ATG10
Application
Application
Western blot analysis of ATG10 in SK-N-SH cell lysate with ATG10 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Application Notes
WB: 0.5 - 2 ug/mL:
Additional Information
Form
Liquid
Storage Instructions
Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.