Application
Various whole cell extracts (40 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG10 antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.Application
ATG10 antibody detects ATG10 protein at cytoplasm in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. ATG10 antibody diluted at 1:500.| Product Name | ATG10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG10 (autophagy related 10)Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | autophagy related 10 APG10L APG10 pp12616 |
|---|---|
| Target | ATG10 |
| Molecular Weight(MW) | 25 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Various whole cell extracts (40 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG10 antibody diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Application
ATG10 antibody detects ATG10 protein at cytoplasm in human ovarian cancer by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. ATG10 antibody diluted at 1:500.| Positive Control | SK-N-SH , LoVo |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
