Application
ATG12 antibody [GT166] detects ATG12 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG12 protein stained by ATG12 antibody [GT166] diluted at 1:1000. Red: Phalloidin, a F-actin marker.Application
ATG12 antibody [GT166] detects ATG12 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE ATG12 antibody [GT166] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT166] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
ATG12 antibody [GT166] detects ATG12 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12% SDS-PAGE ATG12 antibody [GT166] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | ATG12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT166] to ATG12 (ATG12 autophagy related 12 homolog (S. cerevisiae))Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT166 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | ChimpanzeeChimpanzee (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APG12L FBR93 autophagy related 12 HAPG12 APG12 |
|---|---|
| Target | ATG12 |
| Cellular Localization | Cytoplasm |
Application
ATG12 antibody [GT166] detects ATG12 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG12 protein stained by ATG12 antibody [GT166] diluted at 1:1000. Red: Phalloidin, a F-actin marker.
Application
ATG12 antibody [GT166] detects ATG12 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract D. 30 µg NIH-3T3 whole cell lysate/extract E. 30 µg BCL-1 whole cell lysate/extract F. 30 µg Raw264.7 whole cell lysate/extract G. 30 µg C2C12 whole cell lysate/extract 12% SDS-PAGE ATG12 antibody [GT166] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT166] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
ATG12 antibody [GT166] detects ATG12 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 12% SDS-PAGE ATG12 antibody [GT166] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
