Application
ATG14 antibody detects ATG14 protein at cytosol on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. ATG14 antibody dilution: 1:500.Application
Various whole cell and tissue extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.Application
ATG14 antibody detects ATG14 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE ATG14 antibody dilution: 1:1000| Product Name | ATG14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG14 (ATG14 autophagy related 14 homolog (S. cerevisiae))Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ATG14. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATG14L antibody BARKOR antibody KIAA0831 antibody MGC126291 antibody MGC126292 antibody ATG14 antibody autophagy-related protein 14-like protein antibody beclin 1-associated autophagy-related key regulator antibody Beclin 1-Interacting protein anti |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
Application
ATG14 antibody detects ATG14 protein at cytosol on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. ATG14 antibody dilution: 1:500.
Application
Various whole cell and tissue extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
Application
ATG14 antibody detects ATG14 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract 10 % SDS-PAGE ATG14 antibody dilution: 1:1000| Positive Control | HeLa , HepG2 , 293T , RD , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
