Application
ATG16L1 antibody detects ATG16L1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. ATG16L1 antibody dilution: 1:500.Application
ATG16L1 antibody detects ATG16L1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE ATG16L1 antibody dilution: 1:2000Application
ATG16L1 antibody detects ATG16L1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. ATG16L1 antibody dilution: 1:500.Application
ATG16L1 antibody detects ATG16L1 protein at autophagosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATG16L1 protein stained by ATG16L1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
ATG16L1 antibody detects ATG16L1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATG16L1 antibody dilution: 1:500.| Product Name | ATG16L1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG16L1 (autophagy related 16-like 1 (S. cerevisiae))Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG16L1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (93%), Pig (92%), Rhesus Monkey (80%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATG16L WDR30 APG16L IBD10 autophagy related 16-like 1 (S. cerevisiae) ATG16A |
|---|---|
| Target | ATG16L1 |
| Molecular Weight(MW) | 68 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Preautophagosomal structure membrane; Peripheral membrane protein , Cytoplasm , Preautophagosomal structure membrane |
Application
ATG16L1 antibody detects ATG16L1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. ATG16L1 antibody dilution: 1:500.
Application
ATG16L1 antibody detects ATG16L1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract 7.5 % SDS-PAGE ATG16L1 antibody dilution: 1:2000
Application
ATG16L1 antibody detects ATG16L1 protein at cytosol on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. ATG16L1 antibody dilution: 1:500.
Application
ATG16L1 antibody detects ATG16L1 protein at autophagosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ATG16L1 protein stained by ATG16L1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
ATG16L1 antibody detects ATG16L1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATG16L1 antibody dilution: 1:500.| Positive Control | 293T , A431 , HeLa , *MEF |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
