Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded U251 xenograft, using ATG16L1 antibody at 1:500 dilution.| Product Name | ATG16L1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG16L1 (ATG16 autophagy related 16-like 1 (S. cerevisiae))Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG16L1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, BovineMouse (93%), Rat (94%), Pig (93%), Rhesus Monkey (89%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APG16L antibody ATG16A antibody ATG16L antibody FLJ00045 antibody FLJ10035 antibody FLJ10828 antibody FLJ22677 antibody IBD10 antibody WDR30 antibody ATG16L1 antibody WD repeat domain 30 antibody APG16L beta antibody autophagy-related protein 1 |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Preautophagosomal structure membrane; Peripheral membrane protein , Cytoplasm , Preautophagosomal structure membrane |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded U251 xenograft, using ATG16L1 antibody at 1:500 dilution.| Positive Control | NT2D1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
