Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG3 antibody diluted at 1:1000.Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg K562 whole cell lysate/extract B. 30 µg THP-1 whole cell lysate/extract C. 30 µg HL-60 whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:1000Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:1000Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:2000Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:1000| Product Name | ATG3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG3 (ATG3 autophagy related 3 homolog (S. cerevisiae))Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, Rhesus Monkey, BovineChicken (85%), Rhesus Monkey (97%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APG3 antibody APG3-LIKE antibody APG3L antibody DKFZp564M1178 antibody FLJ22125 antibody MGC15201 antibody PC3-96 antibody ATG3 antibody 2610016C12Rik antibody hApg3 antibody ubiquitin-like-conjugating enzyme ATG3 antibody autophagy-related prot |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG3 antibody diluted at 1:1000.
Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg K562 whole cell lysate/extract B. 30 µg THP-1 whole cell lysate/extract C. 30 µg HL-60 whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:1000
Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg BCL-1 whole cell lysate/extract D. 30 µg Raw264.7 whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:1000
Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:2000
Application
ATG3 antibody detects ATG3 protein by Western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract 10 % SDS-PAGE ATG3 antibody dilution: 1:1000| Positive Control | HeLa , K562 , THP-1 , HL-60 , Jurkat , Raji , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
