Application
Immunofluorescence analysis of A549 cells, using ATG4A Antibody. The picture on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ATG4A Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | ATG4A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATG4ACysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. |
| Immunogen | The amino acid range of this immunogen is in aa100-130 from human ATG4A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-P |
| IF: 1:100~1:500 : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AUTlike2 cysteine endopeptidase Autophagyrelated cysteine endopeptidase2 Autophagy-related cysteine endopeptidase 2 Cysteine protease ATG4A Autophagyrelated protein 4 homolog A AUT-like 2 cys AUTlike 2 cysteine endopeptidase EC3.4.22. Autophagy-rel |
|---|---|
| Target | ATG4A |
| Molecular Weight(MW) | 45 kDa (note) |
| Tissue Specificity | ATG4A Antibody detects endogenous levels of total ATG4A protein. |
Application
Immunofluorescence analysis of A549 cells, using ATG4A Antibody. The picture on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ATG4A Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IF: 1:100~1:500 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
