Application
Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 ug/ml.Application
Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 ug/ml.Application
Western blot analysis of ATG4B in 3T3 cell lysate with ATG4B antibody at 1 ug/ml.| Product Name | ATG4B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG4BAutophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Immunogen | ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml. IHC-P: 5 ug/mL. IF: 20 ug/ml: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | autophagy related 4B cysteine peptidase APG4B AUTL1 |
|---|---|
| Target | ATG4B |
| Tissue Specificity | ATG4B antibody is human and mouse reactive. At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins. |
Application
Immunofluorescence of ATG4B in human spleen tissue with ATG4B antibody at 20 ug/ml.
Application
Immunohistochemistry of ATG4B in human spleen tissue with ATG4B antibody at 5 ug/ml.
Application
Western blot analysis of ATG4B in 3T3 cell lysate with ATG4B antibody at 1 ug/ml.| Application Notes | WB: 1 - 2 ug/ml. IHC-P: 5 ug/mL. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
