Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft , using ATG4D antibody at 1:100 dilution.Application
ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200. Blue: Hoechst 33342 staining.Application
ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. ATG4D antibody [N3C3] diluted at 1:500.Application
ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ATG4D antibody [N3C3] diluted at 1:500.| Product Name | ATG4D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATG4D (ATG4 autophagy related 4 homolog D (S. cerevisiae))Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG4D. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (97%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APG4-D antibody APG4D antibody AUTL4 antibody ATG4D antibody AUT-like 4 cysteine endopeptidase antibody autophagin-4 antibody cysteine protease involved in autophagy antibody autophagy-related protein 4 homolog D antibody APG4 autophagy 4 homolog D |
|---|---|
| Target | ATG4D |
| Molecular Weight(MW) | 53 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft , using ATG4D antibody at 1:100 dilution.
Application
ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG4D protein stained by ATG4D antibody [N3C3] diluted at 1:200. Blue: Hoechst 33342 staining.
Application
ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. ATG4D antibody [N3C3] diluted at 1:500.
Application
ATG4D antibody [N3C3] detects ATG4D protein at cytoplasm in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ATG4D antibody [N3C3] diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
