Application
ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 μM chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ATG5 antibody diluted at 1:400.Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: PC-3 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Sample: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min. Green: ATG5 protein stained by ATG5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG5 protein stained by ATG5 antibody diluted at 1:1000. Red: Phalloidin, a F-actin marker.| Product Name | ATG5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG5 (ATG5 autophagy related 5 homolog (S. cerevisiae)) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (81%), Pig (90%), Chicken (87%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APG5 antibody APG5-LIKE antibody APG5L antibody ASP antibody hAPG5 antibody ATG5 antibody autophagy protein 5 antibody apoptosis-specific protein antibody apoptosis specific protein antibody ATG5 autophagy related 5 homolog (S. cerevisiae) antibod |
|---|---|
| Target | ATG5 |
| Molecular Weight(MW) | 32 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
ATG5 antibody detects ATG5 protein by western blot analysis. Un-treated (-) and treated (+, 50 μM chloroquine treatment for 24 hrs) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with ATG5 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ATG5 antibody detects ATG5 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ATG5 antibody diluted at 1:400.
Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: PC-3 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Sample: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) and were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 2 min for min. Green: ATG5 protein stained by ATG5 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
ATG5 antibody detects ATG5 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG5 protein stained by ATG5 antibody diluted at 1:1000. Red: Phalloidin, a F-actin marker.| Positive Control | NT2D1 , PC-3 , HeLa , HeLa (chloroquine treatment for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
