Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9B antibody diluted at 1:1000.Application
ATG9B antibody detects ATG9B protein at cytoplasm by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 500 μM Chloroquine for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG9B protein stained by ATG9B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | ATG9B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG9B (autophagy related 9B)Plays a role in autophagy. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ATG9B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | autophagy related 9B APG9L2 NOS3AS SONE |
|---|---|
| Target | ATG9B |
| Molecular Weight(MW) | 101 kDa (note) (2) |
| Cellular Localization | Cytoplasmic vesicle , autophagosome membrane |
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG9B antibody diluted at 1:1000.
Application
ATG9B antibody detects ATG9B protein at cytoplasm by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 500 μM Chloroquine for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATG9B protein stained by ATG9B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
