Mouse monoclonal antibody [AT2G2] to ATGLThis gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010]
Immunogen
Recombinant human ATGL (30-504aa) purified from E. coli
Key Feature
Clonality
Monoclonal
Isotype
IgG2
Clone Number
AT2G2
Host Species
Mouse
Tested Applications
ELISAIHCIHC-PWB
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.:
Species Reactivity
HumanMouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
WB analysis of mouse kidney lysate (20ug) using ATGL antibody at a dilution of 1:1,000.
Application
IHC-P analysis ofof human breast and prostatic cancer tissue using ATGL antibody at a dilution of 1:25.
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.:
Additional Information
Form
Liquid
Storage Instructions
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Storage Buffer
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.