Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE ATIC antibody diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATIC antibody at 1:100 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATIC antibody at 1:200 dilution.| Product Name | ATIC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATIC (5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase)This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATIC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chicken, BovineMouse (92%), Rat (92%), Pig (92%), Chicken (83%), Bovine (93%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AICAR antibody AICARFT antibody FLJ93545 antibody IMPCHASE antibody PURH antibody ATIC antibody bifunctional purine biosynthesis protein PURH antibody AICARFT/IMPCHASE antibody phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydr |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE ATIC antibody diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATIC antibody at 1:100 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATIC antibody at 1:200 dilution.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
