Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody diluted at 1:500.Application
ATM antibody detects ATM protein by western blot analysis. whole cell extracts and extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody at a dilution of 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATM antibody at 1:500 dilution.| Product Name | ATM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATM (ataxia telangiectasia mutated)The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Dog, Pig, BovineDog (84%), Pig (81%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AT1 antibody ATA antibody ATC antibody ATD antibody ATDC antibody ATE antibody DKFZp781A0353 antibody MGC74674 antibody TEL1 antibody TELO1 antibody ATM antibody AT mutated antibody TEL1 telomere maintenance 1 homolog antibody A-T mutated an |
|---|---|
| Molecular Weight(MW) | 351 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasmic vesicle |
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody diluted at 1:500.
Application
ATM antibody detects ATM protein by western blot analysis. whole cell extracts and extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody at a dilution of 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATM antibody at 1:500 dilution.| Positive Control | HeLa , HeLa NE |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
