Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody by western blot or immunoprecipitation.Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
ATM antibody [2C1] detects ATM protein by western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg PC-3 whole cell lysate/extract C. 30 µg SK-N-SH whole cell lysate/extract 5% SDS-PAGE ATM antibody [2C1] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.| Product Name | ATM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2C1] to ATMThe ATM antibody, clone 2C1, recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation. |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2C1 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PIPWBIHC-RChIP |
| For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa. : |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATD 114480 ATA AT1 ATC ATE DKFZp781A0353 472 ATM TEL1 ATDC Q13315 MGC74674 |
|---|---|
| Target | ATM |
| Molecular Weight(MW) | 370 kDa (note) |
| Cellular Localization | Nucleus |
| Light Chain Type | kappa |
Application
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Detection of human ATM protein using anti-ATM 2C1 monoclonal antibody by western blot or immunoprecipitation.
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
ATM antibody [2C1] detects ATM protein by western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract B. 30 µg PC-3 whole cell lysate/extract C. 30 µg SK-N-SH whole cell lysate/extract 5% SDS-PAGE ATM antibody [2C1] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Human Testis (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.| Positive Control | HeLa , HeLa nuclear extract , NT2D1 , PC-3 , SK-N-SH |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
