Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE diluted at 1:100Application
Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATM antibody at 1:50 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATM antibody at 1:100 dilution.| Product Name | ATM antibody (supernatant) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2C1] to ATM (supernatant)ATM-2C1 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation. |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2C1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PIPWB |
| For WB: Use at 1:100. : |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 114480 ATC DKFZp781A0353 Q13315 MGC74674 ATD ATA AT1 ATE 472 ATM TEL1 ATDC |
|---|---|
| Target | ATM |
| Cellular Localization | Nucleus |
| Light Chain Type | kappa |
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE diluted at 1:100
Application
Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATM antibody at 1:50 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ATM antibody at 1:100 dilution.| Positive Control | Raji and lymphoblastoid nuclear lysate |
|---|---|
| Application Notes | For WB: Use at 1:100. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | TCS containing no preservatives |
