Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ATM antibody at 1:200 dilution.Application
Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ATM antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ATM antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 5% SDS PAGE diluted at 1:1000| Product Name | ATM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3E8] to ATMATM-3E8 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation. |
| Immunogen | Recombinant protein expressed in E. coli corresponding to amino acids 980-1512. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3E8 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| For IHC: Use at a concentration of 2-10 µg/ml. For IP: Use at a concentration of 2-10 µg/ml. For WB: Use at a concentration of 1-2 µg/ml. Predicted moolecular weight: 350kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | ATD 114480 ATA AT1 ATC ATE DKFZp781A0353 472 ATM TEL1 ATDC Q13315 MGC74674 |
|---|---|
| Target | ATM |
| Molecular Weight(MW) | 370 kDa (note) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ATM antibody at 1:200 dilution.
Application
Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ATM antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ATM antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 5% SDS PAGE diluted at 1:1000| Positive Control | Raji, HeLa, or Akata |
|---|---|
| Application Notes | For IHC: Use at a concentration of 2-10 µg/ml. For IP: Use at a concentration of 2-10 µg/ml. For WB: Use at a concentration of 1-2 µg/ml. Predicted moolecular weight: 350kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH7.4, containing no preservatives |
