Application
FACS analysis of HeLa cells using ATP-Citrate Lyase antibody at a dilution of 1:100 (red) or an isotype control (black).Application
WB analysis of K562 and HeLa lysates using ATP-Citrate Lyase antibody at a dilution of 1:1,000.Application
ICC/IF analysis of HeLa cells using ATP-Citrate Lyase antibody at a dilution of 1:150.| Product Name | ATP citrate Lyase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [3D9-E9-H8] to ATP-Citrate LyaseATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| Immunogen | Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3D9-E9-H8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CLATP ATP citrate lyase ATPCL ACL ACLY |
|---|---|
| Target | ATP-Citrate Lyase |
| Molecular Weight(MW) | 120kDa (note) |
| Cellular Localization | Cytoplasm |
Application
FACS analysis of HeLa cells using ATP-Citrate Lyase antibody at a dilution of 1:100 (red) or an isotype control (black).
Application
WB analysis of K562 and HeLa lysates using ATP-Citrate Lyase antibody at a dilution of 1:1,000.
Application
ICC/IF analysis of HeLa cells using ATP-Citrate Lyase antibody at a dilution of 1:150.| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol |
