Application
ELISA analysis of control antigen and antigen using ACLY antibody [5F8D11].Application
WB analysis of human ACLY recombinant protein using ACLY antibody [5F8D11].Application
IHC-P analysis of esophageal cancer tissues using ACLY antibody [5F8D11].Application
WB analysis of (1) HeLa, (2) NIH3T3, (3) C6, (4) COS7 and (5) Raji lysates using ACLY antibody [5F8D11].Application
IHC-P analysis of endometrial cancer tissues using ACLY antibody [5F8D11].Application
FACS analysis of HeLa cells using ACLY antibody [5F8D11] (green) or a negative control (purple).Application
ICC/IF analysis of HeLa cells using ACLY antibody [5F8D11] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).| Product Name | ATP citrate lyase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [5F8D11] to ACLYATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to a region within amino acids 306 and 502 of human ACLY expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5F8D11 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | ATPCL CLATP ACL |
|---|---|
| Target | ACLY |
| Molecular Weight(MW) | 125 kDa (note) |
Application
ELISA analysis of control antigen and antigen using ACLY antibody [5F8D11].
Application
WB analysis of human ACLY recombinant protein using ACLY antibody [5F8D11].
Application
IHC-P analysis of esophageal cancer tissues using ACLY antibody [5F8D11].
Application
WB analysis of (1) HeLa, (2) NIH3T3, (3) C6, (4) COS7 and (5) Raji lysates using ACLY antibody [5F8D11].
Application
IHC-P analysis of endometrial cancer tissues using ACLY antibody [5F8D11].
Application
FACS analysis of HeLa cells using ACLY antibody [5F8D11] (green) or a negative control (purple).
Application
ICC/IF analysis of HeLa cells using ACLY antibody [5F8D11] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer. |
