Application
Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: Jurkat D: Raji 5% SDS PAGE diluted at 1:5000Application
ATP citrate lyase antibody [N1N2], N-term detects ACLY protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACLY protein stained by ATP citrate lyase antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | ATP citrate lyase antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP citrate lyase (ATP citrate lyase)ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ATP citrate lyase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chicken, Sheep, BovineMouse (96%), Rat (96%), Pig (95%), Chicken (85%), Sheep (97%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ACL antibody ATPCL antibody CLATP antibody ACLY antibody ATP citrate synthase antibody ATP-citrate (pro-S-)-lyase antibody ATP-citrate synthase antibody citrate cleavage enzyme antibody ATP citrate lyase antibody |
|---|---|
| Molecular Weight(MW) | 121 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded Hepatoma, using ATP citrate lyase antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: Jurkat D: Raji 5% SDS PAGE diluted at 1:5000
Application
ATP citrate lyase antibody [N1N2], N-term detects ACLY protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ACLY protein stained by ATP citrate lyase antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
