Application
Immunofluorescence of ATP11A in K562 cells with ATP11A antibody at 20 μg/mL.Application
Western blot analysis of ATP11A in K562 cell tissue lysate with ATP11A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunocytochemistry of ATP11A in K562 cells with ATP11A antibody at 10 ug/ml.| Product Name | ATP11A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATP11AATP11A is a widely expressed integral membrane ATPase. ATP11A is probably phosphorylated in its intermediate state and likely drives the transport of ions such as calcium or other molecules across membranes. While the exact molecule ATP11A transports is unknown, increased expression of ATP11A mRNA has been observed in murine leukemia cells made resistant to anti-cancer drugs such as farnesyltransferase inhibitors (FTIs). Furthermore, overexpression of ATP11A provided protection against the FTI SCH66336 while knockdown of ATP11A via siRNA made cells more sensitive to this drug. Other reports suggest that elevated levels of ATP11A mRNA may also be a predictive marker of metastasis in colorectal cancer patients. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATPIH ATP11A ATPIS ATPase class VI type11A ATPase class VI type 11A |
|---|---|
| Target | ATP11A |
| Tissue Specificity | This antibody is specific for ATP11A |
Application
Immunofluorescence of ATP11A in K562 cells with ATP11A antibody at 20 μg/mL.
Application
Western blot analysis of ATP11A in K562 cell tissue lysate with ATP11A antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunocytochemistry of ATP11A in K562 cells with ATP11A antibody at 10 ug/ml.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
