Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000| Product Name | ATP6V0A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP6V0A2 (ATPase, H+ transporting, lysosomal V0 subunit a2)The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain; ATP6V0A2 is a component of the V(0) domain (Smith et al., 2003 [PubMed 14580332]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V0A2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (93%), Rat (94%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | A2 antibody ARCL antibody ATP6A2 antibody ATP6N1D antibody J6B7 antibody RTF antibody STV1 antibody TJ6 antibody TJ6M antibody TJ6S antibody VPH1 antibody WSS antibody ATP6V0A2 antibody A2V-ATPase antibody vacuolar proton translocating ATPase |
|---|---|
| Molecular Weight(MW) | 98 kDa (note) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein , Endosome membrane |
Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000| Positive Control | A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
