Application
Western blot analysis of extracts of various cell lines, using ATP6V1B2 antibody.| Product Name | ATP6V1B2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP6V1B2This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human ATP6V1B2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB 1:500 - 1:2000, IHC 1:20 - 1:200, IP 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | VPP3 ATP6B2 ATP6B1B2 Vma2 ATPase H+ transporting lysosomal 56/58kDa V1 subunit B2 HO57 VATB |
|---|---|
| Target | ATP6V1B2 |
| Molecular Weight(MW) | 57 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using ATP6V1B2 antibody.| Application Notes | WB 1:500 - 1:2000, IHC 1:20 - 1:200, IP 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
