Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000Application
Western blot experiment using ATP6V1E2 Antibody at 1:90000 dilution to detect 100ng of 34kDa recombinant ATP6V1E2 protein antigen.| Product Name | ATP6V1E2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP6V1E2 (ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2)Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. This isoform is essential for energy coupling involved in acidification of acrosome. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1E2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Mouse |
| Predicted Reactivity | Human, Rhesus Monkey, BovineHuman (100%), Rhesus Monkey (97%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATP6E1 antibody ATP6EL2 antibody ATP6V1EL2 antibody MGC9341 antibody VMA4 antibody ATP6V1E2 antibody vacuolar proton pump subunit E 2 antibody V-ATPase subunit E 2 antibody V-type proton ATPase subunit E 2 antibody vacuolar-type proton-translocati |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (2) |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000
Application
Western blot experiment using ATP6V1E2 Antibody at 1:90000 dilution to detect 100ng of 34kDa recombinant ATP6V1E2 protein antigen.| Positive Control | mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
