Application
ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATP6V1H antibody [N3C3] diluted at 1:500.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ATP6V1H antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP6V1H (ATPase, H+ transporting, lysosomal 50/57kDa, V1 subunit H)This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Chicken, BovineZebrafish (91%), Xenopus laevis (94%), Pig (99%), Chicken (96%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MSTP042 antibody NBP1 antibody SFD antibody SFDalpha antibody SFDbeta antibody VMA13 antibody ATP6V1H antibody protein VMA13 homolog antibody V-type proton ATPase subunit H antibody V-ATPase subunit H antibody nef-binding protein 1 antibody vacu |
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| Molecular Weight(MW) | 56 kDa (note) (3) |
Application
ATP6V1H antibody [N3C3] detects ATP6V1H protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATP6V1H antibody [N3C3] diluted at 1:500.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , Raji , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
