| Product Name | ATP7b antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATP7bATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria.Mutations in the ATP7b gene can cause Wilsonis disease, an inherited disorder causing copper poisoning in the brain and liver. |
| Immunogen | A synthetic peptide made to an internal sequence nearer the C-terminus of human ATP7b. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| By Western blot, this antibody recognizes a band at 165 kDa, representing ATP7b protein. This antibody also recognizes a band at ~220 kDa. This antibody may work on endogeneous protein in immunohistochemistry but it has only been tested on transfected ovarian carcinoma cells at a 1:500 dilution. However, GTX30640 may demonstrate less background for this assay.Suggested working dilutions: *Western Blot - 1:1,000 Immunoprecipitation - NDImmunohistochemistry - (see statement above)*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | ATP7b WC1 PWD P35670 WND 540 277900 |
|---|---|
| Target | ATP7b |
| Tissue Specificity | This antibody is specific for ATPb7. |
| Positive Control | HuH7 hepatocarcinoma cell extracts |
|---|---|
| Application Notes | By Western blot, this antibody recognizes a band at 165 kDa, representing ATP7b protein. This antibody also recognizes a band at ~220 kDa. This antibody may work on endogeneous protein in immunohistochemistry but it has only been tested on transfected ovarian carcinoma cells at a 1:500 dilution. However, GTX30640 may demonstrate less background for this assay.Suggested working dilutions: *Western Blot - 1:1,000 Immunoprecipitation - NDImmunohistochemistry - (see statement above)*The investigator should determine the optimal working dilution for a specific application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. |
| Storage Buffer | 0.1 ml of rabbit antisera (1 mg/ml) with 0.1% sodium azide. |
