Application
ATXN2 antibody detects ATXN2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody at a dilution of 1:500.Application
ATXN2 antibody detects ATXN2 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATXN2 protein stained by ATXN2 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | ATXN2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATXN2 (ataxin 2)The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. Defects in this gene are the cause of spinocerebellar ataxia type 2 (SCA2). SCA2 belongs to the autosomal dominant cerebellar ataxias type I (ADCA I) which are characterized by cerebellar ataxia in combination with additional clinical features like optic atrophy, ophthalmoplegia, bulbar and extrapyramidal signs, peripheral neuropathy and dementia. SCA2 is caused by expansion of a CAG repeat in the coding region of this gene. Longer expansions result in earlier onset of the disease. Alternatively spliced transcript variants encoding different isoforms have been identified but their full length sequence has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATXN2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SCA2 ASL13 ataxin 2 TNRC13 ATX2 |
|---|---|
| Target | ATXN2 |
| Molecular Weight(MW) | 140 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
ATXN2 antibody detects ATXN2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ATXN2 antibody at a dilution of 1:500.
Application
ATXN2 antibody detects ATXN2 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATXN2 protein stained by ATXN2 antibody diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
