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For ELISA (Coating): Use at a dilution of 1:100 - 1:500. For ELISA (Primary): Use at a dilution of 1:1,000 - 1:30,000. For ICC: Use at a dilution of 1:200 - 1:2,000. For WB: Use at a dilution of 1:1,000 - 1:30,000. For WB (Chemiluminescent): Use at a dilution of 0.03 - 1 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.:
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