Application
Western blot analysis of extracts of various cell lines, using AUH antibody.Application
Immunohistochemistry of paraffin-embedded rat kidney using AUH antibody at dilution of 1:100 (x400 lens).| Product Name | AUH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AUHThe methylglutaconyl-CoA hydratase, mitochondrial protein binds to the AU-rich element (ARE), a common element found in the 3' UTR of rapidly decaying mRNA such as c-fos, c-myc and granulocyte/ macrophage colony stimulating factor. ARE elements are involved in directing RNA to rapid degradation and deadenylation. AUH is also homologous to enol-CoA hydratase, an enzyme involved in fatty acid degradation, and has been shown to have intrinsic hydratase enzymatic activity. AUH is thus a bifunctional chimera between RNA binding and metabolic enzyme activity. A possible subcellular localization in the mitochondria has been demonstrated for the mouse homolog of this protein which shares 92% identity with the human protein. It has been suggested that AUH may have a novel role as a mitochondrial located AU-binding protein. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015] |
| Immunogen | Recombinant protein of human AUH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AU RNA binding protein/enoyl-CoA hydratase |
|---|---|
| Target | AUH |
Application
Western blot analysis of extracts of various cell lines, using AUH antibody.
Application
Immunohistochemistry of paraffin-embedded rat kidney using AUH antibody at dilution of 1:100 (x400 lens).| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
