Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Aurora A (Phospho-Thr288) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Product Name | Aurora A (phospho Thr288) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Aurora A (Phospho Thr288)Contributes to the regulation of cell cycle progression. Required for normal mitosis. Associates with the centrosome and the spindle microtubules during mitosis and functions in centrosome maturation, spindle assembly, maintenance of spindle bipolarity, centrosome separation and mitotic checkpoint control. Phosphorylates numerous target proteins, including ARHGEF2, BRCA1, KIF2A, NDEL1, PARD3, PLK1 and BORA. Regulates KIF2A tubulin depolymerase activity By similarity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 288 (R-T-T(p)-L-M) derived from Human Aurora A. |
| Modification | p-Thr288 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| Suggested starting dilutions are as follows. IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AIK ARK1 AURA AURORA2 BTAK MGC34538 STK15 STK6 STK7 AURKA protein phosphatase 1 regulatory subunit 47 ARK-1 aurora/IPL1-like kinase serine/threonine kinase 6 breast-tumor-amplified kinase serine/threonine protein kinase 15 aurora kinase A |
|---|---|
| Target | Aurora A (Phospho Thr288) |
| Molecular Weight(MW) | 48 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Aurora A (Phospho-Thr288) Antibody (left) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
