Rabbit polyclonal antibody to Aurora A (phospho Thr288)The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human AuroraA around the phosphorylation site of threonine 288 (R -T-TP-L-M).
Modification
p-Thr288
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-P
IHC: 1:50-100:
Species Reactivity
HumanMouse
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
STK15 STK7 BTAK AURORA2 AURKA ARK1 PPP1R47 AURA AIK aurora kinase A STK6
Target
Aurora A (phospho Thr288)
Tissue Specificity
AuroraA (phospho-Thr288) Antibody detects endogenous levels of Aurora A only when phosphorylated at threonine 288.
Application
Application
IHC-P analysis of human breast carcinoma using Aurora A (phospho Thr288) antibody.
Application Notes
IHC: 1:50-100:
Additional Information
Form
Liquid
Storage Instructions
Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Storage Buffer
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.