Application
Human Tonsil (formalin-fixed, paraffin-embedded) stained with AURKB antibody at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.Application
Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500. Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen| Product Name | Aurora B (phospho Thr232) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to AURKB (phospho Thr232)Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases associate with microtubules during chromosome movement and segregation. Aurora kinase B localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers, and Aurora kinase A (MIM 603072) localizes to centrosomes (Lampson et al., 2004 [PubMed 14767480]).[supplied by OMIM] |
| Immunogen | Synthetic peptide |
| Modification | p-Thr232 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFIHC-PWB |
| WB (1:250 - 1:2000), ELISA (1:10000 - 1:50000), IF, IHC-P (2.5 µg/ml): |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Aurora B aurora related kinase 2 AURORA/IPL1 LIKE KINASE 2 AuroraB aurorarelated kinase 2 AURORA/IPL1LIKE KINASE 2 AURKB (phospho T232) AURKB (phospho Thr232) AURKB (pT232) AURKB (pThr232) AURKB phospho T232 AURKB phospho Thr232 Phospho AURKB ( |
|---|---|
| Target | AURKB |
| Tissue Specificity | aa 223-234 of Human Aurora Kinase B protein. |
Application
Human Tonsil (formalin-fixed, paraffin-embedded) stained with AURKB antibody at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.
Application
Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500. Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen| Application Notes | WB (1:250 - 1:2000), ELISA (1:10000 - 1:50000), IF, IHC-P (2.5 µg/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | +4°C or -20°C, Avoid repeated freezing and thawing. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
