Application
Aurora-C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).Application
In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D).Application
The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4).| Product Name | Aurora C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to IPL-1/STK13/Aurora CThe Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase. |
| Immunogen | Synthetic peptide from N-terminal of human Aurora C |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| For ELISA: Use at a dilution of 1:1,000. For IF: Use at a dilution of 1:100-1:500. For WB: Use at a dilution of 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AIE2 AIK3 ARK3 AurC STK13 aurora-C IPL 1/STK13/Aurora C auroraC IPL1/STK13/Aurora C |
|---|---|
| Target | IPL-1/STK13/Aurora C |
Application
Aurora-C Antibody (C-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Aurora-C antibody detected the Aurora-C protein (arrow).
Application
In the immunofluorescence experiment, staining of HeLa cells expressing GFP-Aurora C is performed at different cellular mitotic stages with the anti-Aurora C Pab as primary antibody (column A), GFP fluorescence (column B), DAPI nuclear staining (column C), and anti-Aurora C merged to DAPI staining (column D).
Application
The anti-Aurora C Pab is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3) or Flag-tagged Aurora C (lane 4).| Positive Control | 293 cell and HeLa cell lysate |
|---|---|
| Application Notes | For ELISA: Use at a dilution of 1:1,000. For IF: Use at a dilution of 1:100-1:500. For WB: Use at a dilution of 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, containing 0.09% sodium azide |
