Application
Autotaxin antibody detects Autotaxin protein at cytoplasm by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: Autotaxin protein stained by Autotaxin antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Autotaxin antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Autotaxin (ectonucleotide pyrophosphatase/phosphodiesterase 2)The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Autotaxin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, BovineMouse (95%), Rat (95%), Xenopus laevis (87%), Chicken (95%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATX antibody ATX-X antibody AUTOTAXIN antibody FLJ26803 antibody LysoPLD antibody NPP2 antibody PD-IALPHA antibody PDNP2 antibody ENPP2 antibody ectonucleotide pyrophosphatase/phosphodiesterase family member 2 antibody phosphodiesterase I/nucleot |
|---|---|
| Molecular Weight(MW) | 99 kDa (note) |
| Cellular Localization | Secreted |
Application
Autotaxin antibody detects Autotaxin protein at cytoplasm by immunofluorescent analysis. Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min. Green: Autotaxin protein stained by Autotaxin antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
