Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AXL antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A549 B: H1299 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
AXL antibody detects AXL protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg whole cell extract of human AXL-transfected 293T cells 7.5% SDS-PAGE AXL antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | AXL antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AXL (AXL receptor tyrosine kinase)The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AXL. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, PigMouse (97%), Rat (97%), Pig (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | JTK11 antibody UFO antibody AXL antibody AXL oncogene antibody tyrosine-protein kinase receptor UFO antibody oncogene AXL antibody AXL transforming sequence/gene antibody AXL receptor tyrosine kinase antibody |
|---|---|
| Molecular Weight(MW) | 98 kDa (note) (4) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using AXL antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A549 B: H1299 5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
AXL antibody detects AXL protein by western blot analysis. A. 30 µg 293T whole cell extract B. 30 µg whole cell extract of human AXL-transfected 293T cells 7.5% SDS-PAGE AXL antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , H1299 , human AXL-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
