Application
Western blot of BaboonA with Baboon antibody Lane 1,2: cells expressing BaboonA Lane 3: mock transfected cells| Product Name | Baboon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BaboonTransmembrane receptor Patched regulates several developmental processes in both invertebrates and vertebrates. In higher vertebrates the patched (Ptc) also acts as a tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc removes the negative interaction of Ptc, and allows the signaling by smoothened. It has been shown that during head morphogenesis in D. melanogaster, Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon, and activation of cell proliferation in the eye antennal disc. The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects. |
| Immunogen | Synthetic peptide: CNKNENMKRIFETDYVPHRLTS, corresponding to N terminal amino acids 200-221 of Drosophilia melanogaster Baboon. The peptide was amidated for conjugation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at an assay dependent dilution. IP: Use at a dilution of >1/200. WB: Use at a dilution of >1/500. Detects a band of approximately 73 kDa Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Drosophila |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATR-1 ATR-I Atf1 Atr45A AtrI BABO Bab CG8224 DmelCG8224 Gprk-3 Gprk3 STK-E l(2)44Fd l(2)k16912 ATR 1 ATR I STK E Gprk 3 ATR1 STKE |
|---|---|
| Target | Baboon |
| Tissue Specificity | The antibody is unique to Baboon protein |
Application
Western blot of BaboonA with Baboon antibody Lane 1,2: cells expressing BaboonA Lane 3: mock transfected cells| Positive Control | D. melanogaster extracts |
|---|---|
| Application Notes | ICC: Use at an assay dependent dilution. IP: Use at a dilution of >1/200. WB: Use at a dilution of >1/500. Detects a band of approximately 73 kDa Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: NoneConstituents: BSA, Glycerol, Ethalene glycol. pH 7.4 |
