Application
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BACE1 antibody [N1C1] diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:2000Application
BACE1 antibody [N1C1] detects BACE1 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. BACE1 antibody [N1C1] dilution: 1:500.Application
BACE1 antibody [N1C1] detects BACE1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. BACE1 antibody [N1C1] dilution: 1:500.| Product Name | BACE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BACE1 (beta-site APP-cleaving enzyme 1)Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BACE1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, Bovine, Guinea pigRat (96%), Pig (99%), Rhesus Monkey (99%), Bovine (98%), Guinea pig (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ASP2 antibody BACE antibody FLJ90568 antibody HSPC104 antibody KIAA1149 antibody BACE1 antibody beta-site amyloid beta A4 precursor protein-cleaving enzyme antibody beta-secretase 1 precursor variant 1 antibody membrane-associated aspartic protease |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (3) |
| Cellular Localization | Membrane |
Application
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BACE1 antibody [N1C1] diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:2000
Application
BACE1 antibody [N1C1] detects BACE1 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. BACE1 antibody [N1C1] dilution: 1:500.
Application
BACE1 antibody [N1C1] detects BACE1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. BACE1 antibody [N1C1] dilution: 1:500.| Positive Control | HeLa , Human brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
