Application
Calibration curve in ELISA for Bacillus anthracis protective antigen (PA) determination using Bacillus anthracis protective antigen antibody clone BAP0105 for capture and clone BAP0106 as a HRP-conjugateApplication
Detection of Bacillus anthracis protective antigen (PA) in Western blotting using Bacillus anthracis protective antigen antibodies: 1. clone BAP0101 ; 2. clone BAP0105 ; clone BAP0106 ; 4. protein mw standard. 10% SDS-PAGE under non-reducing conditions| Product Name | Bacillus anthracis antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [BAP0101] to Bacillus anthracis protective antigenProtective antigen (PA) is one of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. PA binds to a receptor (ATR) in sensitive eukaryotic cells, thereby facilitating the translocation of the enzymatic toxin components, edema factor and lethal factor, across the target cell membrane. PA associated with LF causes death when injected, PA associated with EF produces edema. PA induces immunity to infection with anthrax. Anthrax toxins are composed of three distinct proteins, a protective antigen (PA), a lethal factor (LF) and an edema factor (EF). None of these is toxic by itself. PA+LF forms the lethal toxin (LeTx); PA+EF forms the edema toxin (EdTx). PA-63 forms heptamers and this oligomerization is required for LF or EF binding. Once activated, at low pH, the heptamer undergoes conformational changes and converts from prepore to pore inserted in the membrane, forming cation-selective channels. |
| Immunogen | Highly purified protective antigen of Bacillus anthracis |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | BAP0101 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Recognizes both 83K and truncated 63K forms of PA. Optimal dilutions/concentrations should be determined by the end user.: |
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| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | 2820165 pagA Bacillus anthracis |
|---|---|
| Target | Bacillus anthracis |
| Tissue Specificity | This antibody is not cross-reacting with lethal factor antigen of B. antracis, Y.Pestis, F. Tularensis, and Toxoplasma gondii. |
Application
Calibration curve in ELISA for Bacillus anthracis protective antigen (PA) determination using Bacillus anthracis protective antigen antibody clone BAP0105 for capture and clone BAP0106 as a HRP-conjugate
Application
Detection of Bacillus anthracis protective antigen (PA) in Western blotting using Bacillus anthracis protective antigen antibodies: 1. clone BAP0101 ; 2. clone BAP0105 ; clone BAP0106 ; 4. protein mw standard. 10% SDS-PAGE under non-reducing conditions| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Recognizes both 83K and truncated 63K forms of PA. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.1% sodium azide |
