Application
Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 ug/mL.Application
Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.Application
Immunohistochemical staining of rat thymus using Bad antibody at 2 ug/mL.| Product Name | Bad antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BadThe protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008] |
| Immunogen | Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 0.5 - 2 ug/ml. IHC-P: 2 ug/ml. IF: 10 ug/ml: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BCL2-associated agonist of cell death BAD BBC2 BCL2L8 |
|---|---|
| Target | Bad |
Application
Immunofluorescence of BAD in Rat Thymus cells with BAD antibody at 10 ug/mL.
Application
Western blot analysis of Bad in T24 cell lysates with Bad antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Application
Immunohistochemical staining of rat thymus using Bad antibody at 2 ug/mL.| Application Notes | WB: 0.5 - 2 ug/ml. IHC-P: 2 ug/ml. IF: 10 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
