| Product Name | Bag1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Bag1Bcl-2 proteins are a key regulator of apoptosis that function to either inhibit or promote cell death. Over expression of members such as Bcl-2 and Bcl-xL inhibit the apoptotic process (1,2). Bcl-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag-1, for example, has been found to form a heterodimer with Bcl-2 resulting in the enhancement of the anti-apoptotic effect of Bcl-2 (3,4). Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis (6). Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with Bcl-xL, causing the displacement of Bax. The displacement of Bax allows apoptosis to proceed (7). Bcl-xS, a shorter version of Bcl-xL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that Bcl-xS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway (8,9). |
| Immunogen | Synthetic peptide (Mouse) (C terminal). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| IHC-F:1:10~1:20 IHC-P:1:10~1:50 WB:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Q99933 BAG1 573 601497 |
|---|---|
| Target | Bag1 |
| Tissue Specificity | Reacts with Bag-1 p32, p36, and p50 isoforms |
| Positive Control | HL-60 |
|---|---|
| Application Notes | IHC-F:1:10~1:20 IHC-P:1:10~1:50 WB:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Store this product at 4 °C, do not freeze. The product is stable for one year from the date of shipment. |
| Storage Buffer | PBS with 0.1% sodium azide |
