Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:5000Application
Immunofluorescence analysis of methanol-fixed HeLa, using BAG2 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using BAG2 antibody at 1:500 dilution.| Product Name | BAG2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BAG2 (BCL2-associated athanogene 2)BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BAG2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (96%), Rat (95%), Rhesus Monkey (99%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAG-2 antibody KIAA0576 antibody MGC149462 antibody dJ417I1.2 antibody BAG2 antibody BAG family molecular chaperone regulator 2 antibody BAG-family molecular chaperone regulator-2 antibody bcl-2-associated athanogene 2 antibody dJ417I1.2 (BAG-famil |
|---|---|
| Molecular Weight(MW) | 24 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: A549 12% SDS PAGE diluted at 1:5000
Application
Immunofluorescence analysis of methanol-fixed HeLa, using BAG2 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using BAG2 antibody at 1:500 dilution.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
